Richard A. Padgett, Ph.D.

Professor

Cleveland Clinic Foundation

Research: Mechanism of pre-mRNA splicing

The research in my laboratory is directed at understanding the processes involved in specifying the sites of splicing within a pre-mRNA and the mechanism of the splicing reactions. We focus mainly on the minor or U12-dependent class of introns. Our goals are 1) to determine the similarities and differences between the major and minor splicing pathways, their components and mechanisms; 2) to investigate the underlying mechanism of both splicing systems using tools developed in the first goal; and 3) to investigate the evolutionary origins of the modern structure of eukaryotic genes.

 

Selected Publications

Shukla, G. C. and Padgett, R. A. (2002)
A catalytically active group II intron domain 5 can function in the U12-dependent spliceosome.
Mol. Cell 9, 1145-1150.

Shukla, G. C. and Padgett, R. A. (2004)
U4 small nuclear RNA can function in both the major and minor spliceosomes.
Proc. Natl. Acad. Sci. 101, 93-98.

Shukla, G. C., Cole, A. J., Dietrich, R. D. and Padgett, R. A. (2002)
Domains of human U4atac snRNA required for U12-dependent splicing in vivo.
Nucl. Acids Res. 30, 4650-4657.

Dietrich, R. D., Fuller, J.D. and Padgett, R. A. (2005)
A mutational analysis of U12-dependent splice site dinucleotides.
RNA 11, 1430-1440.